The research team led by Wu Jinshui, a researcher at the Institute of Subtropical Agriculture, Chinese Academy of Sciences, has recently made progress in the study of the abundance and diversity of soil carbon-fixing microorganisms (bacteria and algae) in different subtropical red earths.

The number of soil microorganisms, community structure, and their diversity are affected by many factors such as vegetation types, soil types, and land use patterns. In particular, different types of land use will have a series of effects on soil material composition and nutrient balance, which will lead to changes in the structure and function of soil microbial communities. Soil microorganisms play an important role in promoting soil carbon cycle processes. Therefore, the study of the number, community structure, and diversity of soil carbon-fixing microorganisms (bacteria and algae) under different land use patterns will help to understand soil carbon sequestration capacity and carbon sink effects. It is of great significance to promote the sustainable use of land resources.

Based on this, the team selected soils (forests, rice fields, corn arid lands, and tea garden soils) from four typical land use types in the subtropical hilly region, and used molecular biological methods such as T-RFLP and quantitative PCR. To study the population structure, quantity, and diversity of key carbon-fixing functional microbes (bacteria and algae), and to quantify the quantitative characteristics of the carbon-fixing functional gene (cbbL) encoded by the key enzyme in the Calvin cycle, the RubisCO enzyme. Response and feedback mechanisms for different land use types.

The results showed that different land-use patterns significantly affected the abundance of the functional gene cbbL of the carbon-fixing microorganisms (bacteria and algae). The abundance of cbbL in the paddy soil was the highest, reaching 4.33×108 copies g-1, while the tea plantation had the lowest soil. The cbbL numbers of bacteria and algae were 0.98 x 108 and 0.23 x 106 copies g-1, respectively. T-RFLP analysis showed that under different land use patterns, the dominant population of bacterial cbbL had T-RFs of 60 bp (relative abundance of 12% to 35%) and 128 bp (relative abundance of 23% to 29%). The carbon-fixing bacteria under different land use patterns are mainly facultative autotrophic bacteria, including mycobacteria, Rhodopseudomonas palustris, and Rhodobacter alcaligenes.

However, some less abundant fragments were not evenly distributed in the soil under different land use patterns. Some of the differences reached extremely significant levels, such as T-RF 23 bp, 28 bp, 40 bp, and 51 bp in the soil of tea gardens. Did not appear. The dominant population of algae cbbL was mainly concentrated in T-RFs 123 bp, 132 bp, 148 bp and 382 bp. These fragments are identical to the end fragments of known species of algae, namely, yellow algae, diatoms, and red algae. Moreover, principal component analysis (PCA) showed that soil organic carbon and total nitrogen were the decisive factors affecting the population structure, abundance and diversity of soil bacteria cbbL, and total soil phosphorus content was the main factor affecting algae cbbL. The study deepened the understanding of the biological mechanisms of microbial carbon sequestration in red soil under different land use patterns.

The above research results were published in Microbial Ecology (DOI: 10.1007/s00248-015-0621-8), and the reviewers and editors considered that “Abundance and diversity of CO2-assimilating bacteria and algae within red agricultural soils are modulated by changing management practices”. The research using T-RFLP and quantitative PCR technology is an innovative work to deepen the mechanism of microbial fixation in agricultural soils.... The results are very interesting and useful.” The study was obtained by the Chinese Academy of Sciences and the National Natural Science Foundation of China. Funding.

Synchronizer Assembly

Synchronizer Assembly,Automobile Transmission Synchronizer,Synchronizer Set,Transmission Synchronizer Assembly

ShaoXing Change Auto Synchronizer Ring Co.,Ltd , https://www.sxcjautoparts.com

Posted on